Deletogenic activity of 1,2:7,8-diepoxyoctane in the Salmonella typhimurium tester strain TA102

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• 作者：Picada ; Jaqueline N. ; Khromov-Borisov ; Nikita N. ; et. al.
• 关键词：Deletion mutations ; 1 ; 2 ; 7 ; 8-Diepoxyoctane ; Deletogens ; Salmonella typhimurium ; Tester strain TA102 ; Thiazolylalanine
• 刊名：Mutation Research/Reviews in Mutation Research
• 出版年：1999
• 出版时间：September, 1999
• 年：1999
• 卷：437
• 期：2
• 页码：165-173
• 全文大小：113 K

文摘

1,2:7,8-Diepoxyoctane (DEO), whose deletogenic activity was first demonstrated in ad-3 system of Neurospora crassa and then in different species, has been tested in Salmonella typhimurium tester strain TA102 (hisG428^Ochre). It was confirmed that it is a direct acting mutagen and was found that its activity is stimulated with the S9 mix. Obtained His⁺ revertants were screened on their response to the histidine analog, N-(2-thiazolyl)--alanine (ThiAla). Thirty-two percent of spontaneous and 52 % of DEO-induced revertants were resistant to the analog while no resistance was observed among those induced with 4-nitroquinoline-N-oxide (4NQO). Resistance to ThiAla was interpreted as due to small deletions surrounding the target TAA codon in hisG428^Ochre. Thus, at least two simple test-systems, ad-3 of N. crassa and hisG428^Ochre of S. typhimurium, gave compatible results and might be useful in searching of deletogens.