One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase
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- 作者：Hiroto ; Kikuchi1 ; Hiroaki ; Sakurai1 ; Taizo ; Nagura1 ; Tsutomu ; Aritsuka1 ; Fusao ; Tomita2 ; Atsushi ; Yokota3 ; ; yokota@chem.agr.hokudai.ac.jp
- 关键词：¦Â- ; small-caps"">d-fructofuranose-¦Â- ; small-caps"">d-fructofuranose-2 ; 6&prime ; 6 ; 2&prime ; -dianhydride ; DFA IV ; Levan ; Sucrose ; Levan fructotransferase ; Arthrobacter nicotinovorans ;
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：2010
- 出版时间：March 2010
- 年：2010
- 卷：109
- 期：3
- 页码：240-243
- 全文大小：233 K
文摘
The newly established difructose anhydride IV (DFA IV) production system is comprised of the effective production of levan from sucrose by Serratia levanicum NN, the conversion of the levan into DFA IV by levan fructotransferase from Arthrobacter nicotinovorans GS-9, which is highly expressed in an Escherichia coli transformant, and a practical purification step. The chemical properties of DFA IV were also investigated.