- 作者：Ju Young Yoon ; Hye Yoon Jeong ; Shi Hyoung Kim ; Han Gyung Kim ; Gyeongsug Nam ; Jin Pyo Kim ; Deok Hyo Yoon ; Hyunsik Hwang ; Tae Woong Kimc ; Sungyoul Hong ; Jae Youl Cho
- 关键词：PG ; prostaglandin ; NO ; nitric oxide ; COX ; cyclooxygenase ; iNOS ; inducible NO synthase ; (TNF)-¦Á ; tumor necrosis factor ; TLR ; Toll-like receptors (TLR) ; NF-¦ÊB ; nuclear factor-¦ÊB ; EIA ; enzyme immunoassay ; ELISA ; enzyme-linked immunosorbent assay ; MTT ; 3-4
- 刊名：Journal of Ethnopharmacology
- 出版年：2013
- 出版时间：30 July, 2013
- 年：2013
- 卷：148
- 期：3
- 页码：999-1007
- 全文大小：1019 K
Ethnopharmacological relevance
Evodia lepta (Spreng.) Merr., in the Rutaceae family, is a medicinal plant traditionally used to treat inflammatory symptoms such as in meningitis and hepatitis. However, no study has systematically investigated its anti-inflammatory activities including its molecular mechanism.Materials and methods
The effects of a methanol extract from the roots Evodia lepta (El-ME) were evaluated using lipopolysaccharide (LPS)-treated RAW264.7 cells producing nitric oxide (NO) and prostaglandin E2 (PGE2), and an HCl/ethanol-induced mouse gastritis model. Target molecules were identified by analyzing the activation of transcription factors and their upstream kinases.
Results
El-ME reduced the production of NO and PGE2 from LPS-activated RAW264.7 cells in a dose-dependent manner. El-ME also ameliorated the gastritis symptoms of EtOH/HCl-treated mice. The extract suppressed production of mRNA for the inducible NO synthase (iNOS) and cyclooxygenase (COX)-2; the nuclear translocation of nuclear factor (NF)-¦ÊB; the phosphorylation of upstream kinases that activate NF-¦ÊB; and the kinase activities of Syk and Src.
Conclusion
The anti-inflammatory effects of El-ME might be due to its suppression of Syk/Src and NF-¦ÊB. Considering the in vitro and in vivo efficacy of El-ME, Evodia lepta could be developed into an anti-inflammatory herbal remedy.