Combinatorial library strategy for strong overexpression of the lipase from Geobacillus thermocatenulatus on the cell surface of yeast Pichia pastoris
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- 作者：Ryosuke Yamada ; Yusuke Kimoto ; Hiroyasu Ogino
- 关键词：Cell surface display ; Combinatorial strategy ; Lipase ; Overexpression ; Pichia pastoris ; Yeast
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：113
- 期：Complete
- 页码：7-11
- 全文大小：859 K
文摘
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Enzymes can be displayed on yeast cell surface by cell surface display technique.
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Combinatorial library strategy for the lipase overexpression in yeast was developed.
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GS115/D90 exhibited 5-fold higher lipase activity was obtained by the strategy.
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GS115/D90 comprised ENO1 promoter, GAS1 secretion signal, and GAS1 anchoring protein.
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Overexpression on yeast cell surface could be achieved easily by the strategy.