Cigarette smoke extract induces epithelial-mesenchymal transition of human bladder cancer T24 cells through activation of ERK1/2 pathway

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• 作者：Xin Sun^a ; ¹ ; Qifei Deng^b ; ¹ ; Zhaofeng Liang^c ; Zhiqi Liu^a ; Hao Geng^a ; Li Zhao^a ; Qirui Zhou^d ; Jie Liu^a ; Jiaxing Ma^a ; Daming Wang^a ; Dexin Yu^a ; ^{yudx_urology@126.com} ; Caiyun Zhong^e ; ^{cyzhong@njmu.edu.cn}
• 关键词：CSE ; Cigarette smoke extract ; CS ; Cigarette smoke ; EMT ; Epithelial&ndash ; mesenchymal transition ; MAPK ; Mitogen-activated protein kinase ; ERK1/2 ; extracellular regulated protein kinases 1 and 2 ; AP-1 ; activator protein 1 ; DMSO ; dimethyl sulfoxide ; MTT ; thiazolyl blue tetrazolium bromide ; qRT-PCR ; quantitative reverse transcription-polymerase chain reaction
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：86
• 期：Complete
• 页码：457-465
• 全文大小：3615 K
• 卷排序：86

文摘

Bladder cancer is a common genitourinary malignant disease worldwide. Abundant evidence has shown that cigarette smoke (CS) is a crucial risk factor for bladder cancer. Nevertheless, the mechanism underlying the relationship between cigarette smoking and bladder cancer remains unclear. In the present study, we investigated the effects of cigarette smoke extract (CSE) on mitogen-activated protein kinase (MAPK) pathway activation and EMT alterations in human bladder cancer T24 cells, and the preventive effect of extracellular regulated protein kinases 1 and 2 (ERK1/2) inhibitor U0126 was further examined. Our results illustrated that CSE exposure induced morphological change of human bladder cancer T24 cells, enhanced migratory and invasive capacities, reduced epithelial marker expression and elevated mesenchymal marker expression. Meanwhile, exposure of T24 cells to CSE resulted in activation of ERK1/2 pathway as well as activator protein 1 (AP-1) proteins. Interestingly, treatment with ERK1/2 inhibitor U0126 effectively abrogated CSE-triggered EMT and ERK1/2/AP-1 activation. These findings provide novel insight into the molecular mechanisms of CS-associated bladder cancer and may open up new avenues in the search for potential target of bladder cancer intervention.