Evidence that the kinesin light chain domain contains tetratricopeptide repeat units

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• 作者：Sally Q. Fisher ; Meredith Weck ; Jenna E. Landers ; Jeffrey Emrich ; Shana A. Middleton ; Jordan Cox ; Lisa Gentile ; Carol A. Parish ; ^{cparish@richmond.edu}
• 关键词：Kinesin light chain ; Tetratricopeptide repeat proteins ; Molecular motors
• 刊名：Journal of Structural Biology
• 出版年：2012
• 出版时间：March, 2012
• 年：2012
• 卷：177
• 期：3
• 页码：602-612
• 全文大小：1797 K

文摘

Homology models were built for various length sequences of the kinesin-1 light chain (KLC) domain of Drosophila melanogaster and subjected to 200 ns of all-atom molecular dynamics. We also cloned, expressed and characterized these regions spectroscopically. Results confirm that KLC contains tetratricopeptide repeat units; a regular array of repeating 34-residue helix-loop-helix monomers. Experimental and computational evidence is provided confirming the stability and overall helicity of individual TPR repeats as well as individual TPR units incorporated into a multi-TPR structure.