Decelerated transport and its mechanism of 2,5-hexanedione on middle-molecular-weight neurofilament in rat dorsal root ganglia cells
详细信息 查看全文
- 作者：X.-Y. Hana ; b ; D. Chengc ; F.-Y. Songa ; T. Zenga ; L.-H. Ana ; K.-Q. Xiea ; keqinx@sdu.edu.cn" class="auth_mail
- 关键词：2 ; 5-HD ; 2 ; 5-hexanedione ; ALS ; amyotrophic lateral sclerosis ; BSA ; bovine serum albumin ; DRG ; dorsal root ganglia ; HRP ; horse radish peroxidase ; KHC ; kinesin heavy chain ; NF ; neurofilament ; NF-H ; heavy-molecular-weight neurofilament ; NF-L ; light-molecular-weight neurofilament ; NF-M ; middle-molecular-weight neurofilament ; PBS ; phosphate-buffered saline ; PNS ; peripheral nervous system ; SDS ; sodium dodecyl sulfate
- 刊名：Neuroscience
- 出版年：6 June 2014
- 年：2014
- 卷：269
- 期：Complete
- 页码：192-198
- 全文大小：862 K
文摘
- •
135">Live-cell imaging was first used to study the effect of 2,5-HD on slow axonal transport in vitro.
- •
140">GFP-tagged protein and fluorescence microscope were used to explore NF-M transport.
- •
145">2,5-HD impaired the levels of motor proteins associated with NF-M transport in DRG cells.
- •
150">2,5-HD reduced ATP levels in DRG cells, which provides energy support for NF-M transport.