Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples
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- 作者：Ha Thi Minh Pham ; Kirstin Kunath ; Linda Gehrmann ; Martin Giersberg ; Jochen Tuerk ; Steffen Uhlig ; Gerold Hanke ; Kirsten Simon ; Keith Baronian ; Gotthard Kunze
- 关键词：Microbial biosensor ; Arxula adeninivorans ; Amperometric measurement ; EstraMonitor ; Environmental samples
- 刊名：Sensors and Actuators B: Chemical
- 出版年：2013
- 出版时间：August, 2013
- 年：2013
- 卷：185
- 期：Complete
- 页码：628-637
- 全文大小：1934 K
文摘
The ¡®EstraMonitor¡¯ was developed to provide an automated system for the detection of estrogenic compounds. It can be used for semi-online and continuous monitoring without additional instrumentation. Previously it was used to detect pure estrogenic molecules. In this investigation, the ¡®EstraMonitor¡¯ is used to detect the estrogenic activity in five non-pretreated wastewater samples. The ¡®EstraMonitor¡¯ employs genetically modified Arxula adeninivorans G1212/YRC102-hER¦Á-phyK yeast cells as the detection component. Both immobilized and non-immobilized A. adeninivorans G1212/YRC102-hER¦Á-phyK cells remained fully functional in a wide range of samples, including samples that contained up to 5 % NaCl. The results demonstrate that it is possible to determine the estrogenic effects in environmental samples without the need for sterilization, extraction or concentration. The 17¦Â-estradiol equivalent quotient (bio-EEQ) values in these samples were measured by both biochemical and amperometric methods and were compared to GC-MS analyses (ana-EEQ). The three methods showed a good correlation over a similar detection range.