Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion
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- 作者：Kazukiyo Hayashi ; Shuntaro Yano ; Hiroko Abe ; Yasuko Fujita ; Masao Kishida
- 关键词：Salt-tolerant yeast ; Ethanol-tolerant yeast ; Cell fusion ; Backcross ; Error-prone DNA polymerase ; Saccharomyces cerevisiae
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：114
- 期：4
- 页码：476-478
- 全文大小：360 K
文摘
Salt- and ethanol-tolerant mutants of Saccharomyces cerevisiae, isolated from the uracil-requiring mutant derived from Taiken No. 396 by proofreading-deficient DNA polymerization, showed less growth than their parent strain. The fusants, between these tolerant mutants and the lysine-requiring mutant from Taiken No. 396 obtained by the protoplast fusion, indicated improved growth.