Antifungal, antiaflatoxigenic, and insecticidal efficacy of spearmint (Mentha spicata L.) essential oil

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• 作者：Akash Kedia^a ; Bhanu Prakash^a ; Prashant Kumar Mishra^a ; C.S. Chanotiya^b ; Nawal Kishore Dubey^a ; ^{nkdubey2@rediffmail.com" class="auth_mail} ; ^{nkdubeybhu@gmail.com" class="auth_mail}
• 关键词：Mentha spicata ; Essential oil ; Antifungal ; Aflatoxin B1 ; Callosobruchus chinensis ; Plant-based pesticide
• 刊名：International Biodeterioration and Biodegradation
• 出版年：April, 2014
• 年：2014
• 卷：89
• 期：Complete
• 页码：29-36
• 全文大小：417 K

文摘

This study reports on the chemically characterized essential oil (EO) from Mentha spicata L. which was tested as a plant-based pesticide in because it showed efficacy against food-deteriorating molds, aflatoxin production, lipid peroxidation, and the insect pest Callosobruchus chinensis. The chemical characterization of this EO through GC/GC-MS analysis depicted 13 compounds comprising 97.09% of the EO, carvone being the major component (59.6%). The EO significantly inhibited growth and aflatoxin B₁ production by the toxigenic strain of Aspergillus flavus [LHP(C)-D6] at 1.0 and 0.9聽渭l ml^-1levels, respectively. The EO also had a broad fungitoxic effect against 19 food-deteriorating molds. The oil caused 100% mortality to C.聽chinensis during a fumigation test with an LC₅₀ value of 0.003聽渭l聽ml^鈭? air after 24聽h of treatment and 100% repellency at 0.025聽渭l聽ml^鈭? air concentration. The EO of M.聽spicata at 0.1聽渭l聽ml^鈭? air concentration was recorded as the effective fumigant, showing 98.46% oviposition deterrency, 100% ovicidal activity, 88.84% larvicidal activity, 72.91% pupaecidal activity, and 100% antifeedant activity against C.聽chinensis. The phytotoxicity assay showed 100% germination of EO-treated chickpea seeds. The EO had a low mammalian toxicity with an LD₅₀ of 8342.33聽渭l聽kg^鈭? for oral toxicity on mice. The 50% inhibitory concentration (IC₅₀) value of EO was 18.55聽渭l聽ml^鈭? during a DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay.