Granulocyte colony-stimulating factor fails to enhance leptomeningeal collateral growth in spontaneously hypertensive rats
详细信息 查看全文
- 作者：Yukio Sugiyama ; Yoshiki Yagita ; yagita@neurol.med.osaka-u.ac.jp" class="auth_mail ; yagita@medone.med.osaka-u.ac.jp" class="auth_mail ; Toshiro Yukami ; Akihiro Watanabe ; Naoki Oyama ; Yasukazu Terasaki ; Emi Omura-Matsuoka ; Tsutomu Sasaki ; Hideki Mochizuki ; Kazuo Kitagawa
- 关键词：Arteriogenesis ; Granulocyte colony-stimulating factor ; Brain hypoperfusion
- 刊名：Neuroscience Letters
- 出版年：3 April, 2014
- 年：2014
- 卷：564
- 期：Complete
- 页码：16-20
- 全文大小：1305 K
文摘
The promotion of collateral artery growth is an attractive approach for the treatment of chronic brain hypoperfusion due to occlusive artery disease. We previously reported that hypertension impaired the collateral artery growth of leptomeningeal anastomoses after brain hypoperfusion. Granulocyte colony-stimulating factor (G-CSF) enhances arteriogenesis in a mouse model via a mechanism involving monocyte/macrophage mobilization. However, the arteriogenic effect of G-CSF in hypertension remains unknown. In the present study, we tested whether G-CSF affected collateral artery growth in both normotensive and hypertensive model rat. Left common carotid artery (CCA) occlusion was performed to induce hypoperfusion in the brains of Wistar rats and spontaneously hypertensive rats (SHR). G-CSF was administered subcutaneously for 5 consecutive days. The superficial angioarchitecture of the leptomeningeal anastomoses and the circle of Willis after CCA occlusion and G-CSF treatment were visualized by latex perfusion. Circulating blood monocytes and CD68-positive cells, which represented the macrophages on the dorsal surface of the brain, were counted. G-CSF enhanced leptomeningeal collateral growth in Wistar rats, but not in SHR. G-CSF increased circulating blood monocytes in both Wistar rats and SHR. The number of CD68-positive cells on the dorsal surface of the brain was increased by G-CSF in Wistar rats, but not in SHR. The increase in macrophage accumulation correlated with the observed arteriogenic effects. In conclusion, G-CSF promotes collateral artery growth in the normotensive model rat, but not in the hypertensive model rat.