Cell fusion assay by expression of respiratory syncytial virus (RSV) fusion protein to analyze the mutation of palivizumab-resistant strains
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- 作者：Yosuke Yasui
- 关键词：Respiratory syncytial virus ; F protein ; G protein ; SH protein ; Cell fusion ; Palivizumab resistant strain
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：48-55
- 全文大小：2162 K
文摘
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Human respiratory syncytial virus (RSV) causes lower respiratory tract illness in infants.
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RSV F expression plasmid induced syncytia in Vero cells under T7 RNA polymerase.
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Co-expression of RSV F and SH proteins induced large syncytia.
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Single amino acid substitution at 272 or 275 in RSV F protein conferred resistance to palivizumab.
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Single amino acid substitution at 276 in RSV F protein conferred no resistance to palivizumab.