cdk5 Expression and association with p35^nck5a in early stages of rat cerebellum neurogenesis; tyrosine dephosphorylation and activation in post-mitotic neurons

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• 作者：Lazaro ; Jean-Bernard ; Kitzmann ; Magali ; Cavadore ; Jean-Claude ; Muller ; Yves ; Clos ; Jean ; Fernandez ; Anne ; et. al.
• 关键词：cdk5 ; p35 ; Kinase activation ; Cerebellum post-natal neurogenesis
• 刊名：Neuroscience Letters
• 出版年：1996
• 出版时间：October 25, 1996
• 年：1996
• 卷：218
• 期：1
• 页码：21-24
• 全文大小：512 K

文摘

We have examined the expression of cyclin dependent kinase (cdk) 5 protein kinase and p35^nck5a, its activator subunit, during postnatal neurogenesis in rat cerebellum, using mono-specific antibodies. Both cdk5 and p35^nck5a are present and associated in proliferative stages, although cdk5-p35 kinase activity is barely detectable. Cdk5-p35 activity, but not the expression of either subunit, increases up to 6-fold during neuronal differentiation. Since we observe that cdk5 is phosphorylated on tyrosine in proliferative, but not in post-mitotic stages, we suggest that post-translational regulatory mechanisms control cdk5-p35 protein kinase activity during neurogenesis.