A novel papillation assay for the identification of genes affecting mutation rate in Pseudomonas putida and other pseudomonads
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- 作者：Mari Tagel ; Kairi Tavita ; Rita Hõ ; rak ; Maia Kivisaar ; Heili Ilves ; heili.ilves@ut.ee" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pseudomonads ; Papilla ; Mutator ; Mutagenesis ; truA ; gacS
- 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：790
- 期：Complete
- 页码：41-55
- 全文大小：1884 K
文摘
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A novel sucrose-based papillation assay for detection of mutations.
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Visual inspection of mutations as mucous papillae on the surface of colonies.
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The novel assay is applicable in wide range of pseudomonads.
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Numerous genes affecting mutation frequency in P. putida were revealed.