Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior

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• 作者：Daiki Fujise ; Kiyomi Tsuji ; Naoko Fukushima ; Kohei Kawai ; Ken-ichi Harada
• 关键词：Cyanobacteria ; Volatile organic compounds ; Analysis ; β ; -Cyclocitral ; 2-Methyl-1-butanol ; 3-Methyl-1-butanol ; SPME
• 刊名：Journal of Chromatography A
• 出版年：2010
• 出版时间：24 September 2010
• 年：2010
• 卷：1217
• 期：39
• 页码：6122-6125
• 全文大小：195 K

文摘

In order to fully understand the role of volatile organic compounds (VOCs) under natural conditions, an adaptable analytical method was developed as the first step. β-Ionone, β-cyclocitral, 2-methyl-1-butanol and 3-methyl-1-butanol were simultaneously analyzed in addition to geosmin and 2-MIB using GC/MS with SPME. The slight modification of a known method allowed the simultaneous detection and quantification of these VOCs. The SIM of the 3-methyl-1-butanol was always accompanied by a shoulder peak, suggesting the presence of two compounds. In order to separate both compounds, the GC/MS conditions were optimized, and the additional peak was identified as 2-methyl-1-butanol by direct comparison of the authentic compound, indicating that the Microcystis strain always produces a mixture of 2-methyl-1-butanol and 3-methyl-1-butanol. Furthermore, it was found that 2-methyl-1-butanol and 3-methyl-1-butanol were predominant in the dissolved fractions. β-Cyclocitral was easily oxidized to provide the oxidation product, 2,6,6-trimethylcyclohexene-1-carboxylic acid, which causes the blue color formation of cyanobacteria as a consequence of acid stress. The intact acid could be satisfactorily analyzed using the usual GC/MS without derivatization.