Hollow carbon spheres trigger inflammasome-dependent IL-1β secretion in macrophages

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• 作者：Fernando T. And&oacute ; nup>aup>up> ; up>up>bup>up> ; up>up>cup>up> ; up>up>1up> ; Sourav P. Mukherjeeup>aup>up> ; up>up>1up> ; Isabel Gessnerup>dup> ; Laura Wortmannup>dup> ; Lisong Xiaoup>dup> ; Kjell Hultenbyup>eup> ; Anna A. Shvedovaup>fup>up> ; up>up>gup> ; Sanjay Mathurup>dup> ; Bengt Fadeelup>aup>up> ; up>up>Bengt.Fadeel@ki.seup>
• 关键词：ASC ; apoptosis-associated speck-like protein ; CBMs ; carbon-based nanomaterials ; HCS ; hollow carbon spheres ; HMDM ; human monocyte-derived macrophages ; LAL ; limulus amoebocyte lysate ; LPS ; lipopolysaccharide ; NAC ; N-acetyl-cysteine ; NLRP3 ; NLR (nucleotide-binding domain and leucine-rich repeat containing) family pyrin domain containing 3 (also known as NALP3 ; for NACHT ; LRR and PYD domain-containing protein 3)
• 刊名：Carbon
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：243-251
• 全文大小：1585 K
• 卷排序：113

文摘

It is disputed whether inflammasome activation leading to secretion of pro-inflammatory interleukin (IL)-1β in macrophages transpires independently of cell death or whether the two processes are linked. Here, we synthesized hollow carbon spheres (HCS) and investigated their effects on primary human monocyte-derived macrophages (HMDM); short (500 nm) non-functionalized single-walled carbon nanotubes (SWCNT) were included for comparison. HCS (250 nm) were readily taken up by HMDM and induced ROS production, but did not trigger a loss of cell viability. However, a dose- and time-dependent release of IL-1β was detected in lipopolysaccharide (LPS)-primed macrophages upon exposure to HCS, while SWCNT-induced secretion of IL-1β was less pronounced. HCS-triggered IL-1β secretion was cathepsin B- and caspase-1-dependent, and was accompanied by a reduction in intracellular K+. Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N-acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. HCS also triggered IL-1β release in LPS-primed THP.1 cells, but not in THP.1 cells with silencing of ASC, NLRP3, or caspase-1 expression, providing evidence that IL-1β was elicited through NLRP3 inflammasome activation. These studies shed light on the effects of HCS on primary macrophages, and show that spherical carbon-based nanoparticles are potent inflammasome activators.