Association between P-selectin glycoprotein ligand-1 and pathogenesis in acute coronary syndrome assessed by optical coherence tomography

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• 作者：Yuichi Ozaki ; Toshio Imanishi ; ^{t-imani@wakayama-med.ac.jp" class="auth_mail} ; Ikuko Teraguchi ; Tsuyoshi Nishiguchi ; Makoto Orii ; Yasutsugu Shiono ; Kunihiro Shimamura ; Kohei Ishibashi ; Takashi Tanimoto ; Takashi Yamano ; Yasushi Ino ; Tomoyuki Yamaguchi ; Takashi Kubo ; Takashi Akasaka
• 关键词：Acute coronary syndrome ; Optical coherence tomography ; PSGL-1 ; Thrombus
• 刊名：Atherosclerosis
• 出版年：April, 2014
• 年：2014
• 卷：233
• 期：2
• 页码：697-703
• 全文大小：452 K

文摘

Objective

Although monocytes appear to be actively involved in the onset of acute coronary syndrome (ACS), they are heterogenous in human peripheral blood. How up-regulation of monocyte subsets leads to coronary plaque rupture followed by thrombus formation remains unclear. Recent studies have shown that P-selectin glycoprotein ligand-1 (PSGL-1) is involved in monocyte activation in patients with thrombus formation. We therefore investigated the relationship between the expression of PSGL-1 on monocyte subsets and thrombus formation using frequency-domain optical coherence tomography (FD-OCT) in patients with ACS.

Methods

We enrolled a total of 100 individuals in this study: patients with acute myocardial infarction (AMI, n聽=聽25), unstable angina pectoris (UAP, n聽=聽20), or stable angina pectoris (n聽=聽35) who underwent coronary angiography, and control subjects (n聽=聽20). Three monocyte subsets (CD14⁺⁺CD16^{鈭?/sup>, CD14++CD16+, and CD14+CD16+) and the expression of PSGL-1 were measured by flow cytometry. In patients with AMI and UAP, FD-OCT was performed before percutaneous coronary intervention.}

Results

Circulating peripheral CD14⁺⁺CD16⁺ monocytes expressed PSGL-1 more frequently than CD14⁺⁺CD16^{鈭?/sup> and CD14+CD16+ monocytes in patients with ACS. The expression of PSGL-1 on circulating peripheral CD14++CD16+ monocytes was significantly elevated in patients with AMI compared with the other 3 groups. Moreover, the expression levels of PSGL-1 on CD14++CD16+ monocytes were significantly higher in patients with plaque rupture or intracoronary thrombus assessed by FD-OCT.}

Conclusion

Up-regulation of PSGL-1 on CD14⁺⁺CD16⁺ monocytes may be a crucial role in plaque rupture and thrombus formation.