In vitro metabolic engineering for the salvage synthesis of NAD+
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- 作者：Kohsuke Hondaa ; b ; honda@bio.eng.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Naoya Haraa ; Maria Chenga ; b ; Anna Nakamuraa ; Komako Mandaia ; Kenji Okanoa ; Hisao Ohtakea
- 关键词：NAD+ ; Thermophilic enzyme ; In vitro metabolic pathway
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：114-120
- 全文大小：543 K
文摘
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In vitro salvage synthesis of NAD+ from its thermal degradants was achieved.
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The in vitro synthetic pathway was constructed using eight thermophilic enzymes.
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NAD+ concentration was kept constant at 60 °C for 15 h through the in vitro pathway.