Greater amberjack FSHR and LHR responded strongly to their own ligands.
Competitive ELISAs were developed for greater amberjack FSH and LH.
Pituitary fshb, ovarian fshr, and plasma E2 increased during ovarian development.
Plasma FSH increased during the post-spawning period in female greater amberjack.
Pituitary lhb, ovarian lhr, and plasma LH were high during the spawning period.