Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation

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• 作者：Mehmet Ö ; ztü ; rk ; Ufuk Kolak ; Gü ; laç ; tı ; Topç ; u ; Sevil Ö ; ksü ; z ; M. Iqbal Choudhary
• 关键词：Lamiaceae ; Micromeria cilicica ; Antioxidant activity ; Anticholinesterase activity ; Piperitone 7-O-β ; -d-glucoside ; Isothymonin 4′ ; -methyl ether
• 刊名：Food Chemistry
• 出版年：2011
• 出版时间：1 May 2011
• 年：2011
• 卷：126
• 期：1
• 页码：31-38
• 全文大小：250 K

文摘

From the acetone extract of Micromeria cilicica, two new (1 and 2) and five known (3–7) compounds were obtained through radical-scavenging activity guided isolation. Structures of the compounds were identified as piperitone 7-O-β-d-glucoside (1), isothymonin 4′-methyl ether (2), sudachitin (3), isomucronulatol (4), rutin (5), ursolic acid (6) and saccharose (7), based on UV, 1D-and 2D-NMR and mass spectroscopic techniques. The antioxidant potentials of the extract and the isolated compounds were established by using three radical-scavenging assays, namely, DPPH scavenging, scavenging and ABTS⁺ scavenging, besides β-carotene bleaching assay. Particularly, the acetone extract showed a strong inhibition of lipid peroxidation by β-carotene bleaching assay, with a result close to that of (+)-catechin. Among the pure compounds, rutin (5) showed the strongest lipid peroxidation inhibition and antiradical activity while both sudachitin (3) and isomucronulatol (4) exhibited noticeable ABTS⁺ scavenging activity. The anticholinesterase activity of the compounds (1–7) was also determined. Against acetylcholinesterase, they exhibited weak inhibition while compounds 3, 4 and 6 exhibited moderate inhibition against butyrylcholinesterase.