Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro

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• 作者：Valery Grinevich ; Alex ; er Kolleker ; Marina Eliava ; Naoki Takada ; Hiroshi Takuma ; Yugo Fukazawa ; Ryuichi Shigemoto ; Dietmar Kuhl ; Jack Waters ; Peter H. Seeburg ; Pavel Osten
• 关键词：Immediate early genes ; Arc ; Arg3.1 ; Green fluorescent protein (GFP) ; Transgenic mice ; Synaptic plasticity ; Neural circuits ; Stress
• 刊名：Journal of Neuroscience Methods
• 出版年：2009
• 出版时间：30 October 2009
• 年：2009
• 卷：184
• 期：1
• 页码：25-36
• 全文大小：3163 K

文摘

The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.