Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays
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- 作者：Andrey Semenyuk ; Matilda Ahnfelt ; Camilla Estmer Nilsson ; Xiao Yong Hao ; Andras Fö ; ldesi ; Yu-Shu Kao ; Hong-Huei Chen ; Wei-Chen Kao ; Konan Peck and Marek Kwiatkowski
- 关键词：Oligonucleotide ; Synthesis ; Purification ; Cartridge ; Microarray
- 刊名：Analytical Biochemistry
- 出版年：2006
- 出版时间：1 September 2006
- 年：2006
- 卷：356
- 期：1
- 页码：132-141
- 全文大小：361 K
文摘
A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.