Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinase

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• 作者：Andrea Varga ; Beá ; ta Flachner ; Peter Konarev ; É ; va Grá ; czer ; Judit Szabó ; Dmitri Svergun ; Pé ; ter Zá ; vodszky and Má ; ria Vas
• 关键词：Domain movement ; Main hinge ; Substrate effects ; Phosphoglycerate kinase ; Small-angle X-ray scattering
• 刊名：FEBS Letters
• 出版年：2006
• 出版时间：15 May 2006
• 年：2006
• 卷：580
• 期：11
• 页码：2698-2706
• 全文大小：914 K

文摘

Closure of the two domains of 3-phosphoglycerate kinase, upon substrate binding, is essential for the enzyme function. The available crystal structures cannot provide sufficient information about the mechanism of substrate assisted domain closure and about the requirement of only one or both substrates, since lattice forces may hinder the large scale domain movements. In this study the known X-ray data, obtained for the open and closed conformations, were probed by solution small-angle X-ray scattering experiments. The results prove that binding of both substrates is essential for domain closure. Molecular graphical analysis, indeed, reveals formation of a double-sided H-bond network, which affects substantially the shape of the main molecular hinge at β-strand L, under the concerted action of both substrates.