Rebinding and recognition properties of protein-macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres

详细信息    查看全文

• 作者：Kongyin Zhao ; Guoxiang Cheng ; Jianjun Huang ; Xiaoguang Ying
• 关键词：Phosphate ; Alginate ; Hybrid polymer microspheres ; Surface molecular imprinting ; Imprinting efficiency (IE) ; Recognition
• 刊名：Reactive and Functional Polymers
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：68
• 期：3
• 页码：732-741
• 全文大小：650 K

文摘

Calcium phosphate/alginate hybrid polymer microspheres with bovine serum albumin (BSA) embedded and coated on the surface were prepared with sodium alginate (SA) and (NH₄)₂HPO₄ by using CaCl₂ as the gelling agent in an inverse suspension. The morphology and pore structure of imprinted hybrid polymer microspheres was observed by optical microscopy, ESEM, TEM and SEM. Infrared spectrum analysis demonstrated that new hybrid components were produced in the polymer microspheres. Rebinding dynamic and thermodynamic behaviors of the two imprinted microspheres were evaluated, resulting in a higher BSA affinity for imprinted microspheres relative to non-imprinted ones. The factors influencing the imprinting efficiency (IE) of imprinted microspheres were also studied, including the concentration of CaCl₂, template content and pH in rebinding solution, etc. All the results indicated that the surface imprinted microspheres exhibited better imprinting efficiency and higher rebinding capacity. Selectivity tests showed that the imprinted beads exhibited good recognition properties for the template protein versus others.