Expression profile of cytokine genes in Fugu monocytes stimulated with TLR agonists

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• 作者：Hiroki Korenaga ; Ryusuke Nagamine ; Masahiro Sakai ; Tomoya Kono
• 关键词：LPS ; lipopolysaccharide ; polyI ; C ; polyinosinic&ndash ; polycytidylic acid ; RT ; reverse transcription ; PCR ; polymerase chain reaction ; qPCR ; quantitative real-time PCR
• 刊名：International Immunopharmacology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：17
• 期：2
• 页码：390-399
• 全文大小：1243 K

文摘

Monocytes are antigen-presenting cells (APCs) in mammals. Antigen presentation by monocytes via costimulatory molecules was recently confirmed in the Japanese pufferfish Fugu rubripes (also known as Takifugu rubripes) (Sugamata et al., 2009). However, no detailed investigations examining how cytokine gene expression regulates the activation/differentiation of these cells have been conducted to date. Therefore, in this study, the expression of cytokine genes in Fugu monocytes stimulated with TLR agonists (LPS, polyI:C, and IMQ) was profiled. First, the morphological changes and phagocytic activity of stimulated monocytes were examined. At 5 days post-stimulation, the ratio of activated to inactivated monocytes increased (as determined by FCM analysis), and the phagocytic activity of the cells was higher (5-15 % ) than that of nonactivated cells. Analysis of cytokine gene expression in stimulated monocytes revealed that the genes encoding CSF-1b and IFN-¦Ã are important cytokines in activation/differentiation of monocytes/macrophages, and that genes encoding inflammatory cytokines such as IL-1¦Â, IL-6, IL-18, and TNF-¦Á are upregulated by stimulation. The present study revealed the types of cytokines expressed in Fugu monocytes stimulated with TLR agonists.