Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK

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• 作者：Kosuke Nakamura^a ; ^{kosnakamura@nihs.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Kazunari Kondo^a ; ^{kondo@nihs.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Hiroshi Akiyama^a ; Takumi Ishigaki^a ; Akio Noguchi^a ; Hiroshi Katsumata^b ; Kazuto Takasaki^b ; Satoshi Futo^b ; Kozue Sakata^a ; Nozomi Fukuda^a ; Junichi Mano^c ; Kazumi Kitta^c ; Hidenori Tanaka^d ; Ryo Akashi^d ; Tomoko Nishimaki-Mogami^a
• 关键词：Genetically modified ; Real-time PCR ; Carica papaya L. ; Validation data
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：1165-1170
• 全文大小：382 K

文摘

This article is referred to research article entitled “Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method” (Nakamura et al., 2016) [1].

Real-time polymerase chain reaction (PCR) detection method for unauthorized genetically modified (GM) papaya (Carica papaya L.) line PRSV-YK (PRSV-YK detection method) was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976). Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided.