Improved method for the isolation and purification of human islets of Langerhans using Liberase™ enzyme blend
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- 作者：Olack ; Barbara J. ; Swanson ; Carol J. ; Howard ; Todd K. ; Mohanakumar ; T.
- 关键词：human islet isolation ; collagenase ; islet purification ; BMI ; body mass index ; BSA ; bovine serum albumin ; CIT ; cold ischemia time ; COD ; cause of death ; FDA ; fluorescein diacetate ; HBSS ; Hank’ ; s balanced salt solution ; IE ; islet equivalents ; KRBB ; Kreb&
- 刊名：Human Immunology
- 出版年：1999
- 出版时间：December, 1999
- 年：1999
- 卷：60
- 期：1
- 页码：1303-1309
- 全文大小：297 K
文摘
To determine the effects of procedural modifications, 23 human islet isolations were analyzed. Isolations were divided into two groups based on the enzyme used. The influence of Liberase™, with an improved method of mechanical disassociation of pancreas, was compared to an automated method using Sevac collagenase. Pancreases were processed within 10 h of cross clamping. Following ductal injection of the enzyme, tissue was placed in the digestion chamber for disassociation. Purification was accomplished using a COBE 2991 cell processor and continuous gradients of Hypaque EuroFicoll.