Counting Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during Viral Infection

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• 作者：Kaja Murali-Krishna ; John D Altman ; M Suresh ; David J.D Sourdive ; Allan J Zajac ; Joseph D Miller ; Jill Slansky ; Rafi Ahmed
• 刊名：Immunity
• 出版年：1998
• 出版时间：1 February 1998
• 年：1998
• 卷：8
• 期：2
• 页码：177-187
• 全文大小：253 K

文摘

Viral infections induce extensive T cell proliferation in vivo, but the specificity of the majority of the responding T cells has not been defined. To address this issue we used tetramers of MHC class I molecules containing viral peptides to directly visualize antigen-specific CD8 T cells during acute LCMV infection of mice. Based on tetramer binding and two sensitive assays measuring interferon-γ production at the single-cell level, we found that 50 % –70 % of the activated CD8 T cells were LCMV specific (2 × 10⁷ virus-specific cells/spleen). Following viral clearance, antigen-specific CD8 T cell numbers dropped to 10⁶ per spleen and were maintained at this level for the life of the mouse. Upon rechallenge with LCMV, there was rapid expansion of memory T cells, but after infection with the heterologous vaccinia virus there was no detectable change in the numbers of LCMV-specific memory CTL. Therefore, much of the CD8 T cell expansion seen during viral infection represents antigen-specific cells and warrants a revision of our current thinking on the size of the antiviral response.