Glucagon-like peptide-1 receptor expression in Xenopus oocytes stimulates inositol trisphosphate-dependent intracellular Ca²⁺ mobilization

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• 作者：Gromada ; Jesper ; Anker ; Charlotte ; Bokvist ; Krister ; Knudsen ; Lotte B. ; Wahl ; Philip
• 关键词：Glucagon-like peptide-1 ; Calcium ; Inositol trisphosphate ; Xenopus oocyte ; Cyclic adenosine monophosphate ; [Ca²⁺]_i ; intracellular free Ca²⁺ concentration ; GLP-1 ; glucagon-like peptide-1 ; GLP-1(7&ndash ; 36)amide ; glucagon-lik
• 刊名：FEBS Letters
• 出版年：1998
• 出版时间：March 27, 1998
• 年：1998
• 卷：425
• 期：2
• 页码：277-280
• 全文大小：144 K

文摘

The signal transduction pathway of the cloned human glucagon-like peptide-1 (GLP-1) receptor was studied in voltage-clamped Xenopus oocytes. Binding of GLP-1(7–36)amide was associated with cAMP production, increased [Ca²⁺]_i and activation of Ca²⁺-dependent Cl⁻ current. The effect of GLP-1(7–36)amide reflects intracellular Ca²⁺ mobilization and was suppressed by injection of the Ca²⁺ chelator BAPTA and the inositol trisphosphate receptor antagonist heparin. The responses were not mimicked by the adenylate cyclase activator forskolin and unaffected by the protein kinase A (PKA) inhibitor Rp-cAMPS. We conclude that GLP-1 receptor expression in Xenopus oocytes evokes inositol trisphosphate-dependent intracellular Ca²⁺ mobilization independent of the cAMP/PKA signaling pathway.