Diversity of SREBP-1 gene expression in rat adipose tissue depots in response to refeeding after food restriction

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• 作者：Stelmanska ; Ewa ; Sucajtys-Szulc ; Elzbieta ; Korczynska ; Justyna ; Adrych ; Krystian ; Swierczynski ; Julian
• 关键词：Sterol regulatory element binding protein-1 ; Malic enzyme ; Restricted diet ; White adipose tissue ; Rat
• 刊名：BBA - Molecular and Cell Biology of Lipids
• 出版年：2005
• 出版时间：April 15, 2005
• 年：2005
• 卷：1733
• 期：2-3
• 页码：130-136
• 全文大小：161 K

文摘

The SREBP-1c mRNA level and precursor (microsomal) form of SREBP-1 abundance were significantly higher in epididymal and perirenal than in subcutaneous white adipose tissue of control rats. Moreover, the SREBP-1c mRNA level and an amount of precursor form of SREBP-1 were significantly higher in the epididymal and perirenal white adipose tissue of rats maintained on restricted diet and refed ad libitum for 48 h as compared to the control animals. No significant effects of food restriction/refeeding on SREBP-1c mRNA level and an amount of precursor form of SREBP-1 were found in subcutaneous white adipose tissue. The mature (nuclear) form of SREBP-1 was significantly increased in the epididymal, perirenal and subcutaneous white adipose tissue of the food restricted/refed animals. The activity, protein level and the mRNA abundance of malic enzyme (one of the target genes for SREBP-1) increased significantly in the epididymal, perirenal and subcutaneous white adipose tissue of the food restricted/refed rats as compared to the control animals, however the increase in perirenal and epididymal was higher than in the subcutaneous white adipose tissue.

The results presented suggest that SREBP-1c is differently expressed in various rat white adipose tissue depots both under basal (control) and dieting conditions.