High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells
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- 作者：Sergi Regot1 ; sregot@stanford.edu" class="auth_mail ; Jacob J. Hughey1 ; Bryce T. Bajar1 ; Silvia Carrasco1 ; Markus W. Covert1 ; mcovert@stanford.edu" class="auth_mail
- 刊名：Cell
- 出版年：19 June 2014
- 年：2014
- 卷：157
- 期：7
- 页码：1724-1734
- 全文大小：3958 K
文摘
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Kinase substrates were engineered to convert phosphorylation to localization changes
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Genetically encoded biosensors are named kinase translocation reporters (KTR)
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A mathematical model calculates active kinase concentrations from KTR data
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KTRs enable the generation of unprecedented multidimensional data in single cells