Over-expressing transporters associated with antigen processing increases antitumor immunity response in prostate cancer

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• 作者：Tao Qiu¹ ; Lei Wang¹ ; Xiu-heng Liu ; ^{mdliuxh@yahoo.cn} ; Xiao-dong Weng ; You-lin Kuang ; Zhi-yuan Chen ; Hui Chen ; Heng-cheng Zhu
• 关键词：TAP1 ; transporters associated with antigen processing ; DC ; dendritic cell ; APM ; Antigen Processing Machinery Lenti ; Lentivirus ; PC ; prostate cancer ; TAA ; tumour associated antigens ; PLC ; peptide-loading complex
• 刊名：Cellular Immunology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：279
• 期：2
• 页码：167-173
• 全文大小：1026 K

文摘

As we know, prostate cancer down-regulates expression of HLA-1 Antigen Processing Machinery (APM) and has defects in the antigen presentation pathway. In vitro, the prostate cancer cell (PC-3 cells) infected with Lentivirus TAP1 can efficiently over-express TAP1 and Tapasin, and HLA-1 was also up-regulated on the surface of the infected cells. The lentivirus TAP1 infection increased the apoptosis rate of PC-3 cells. In addition, with the co-cluture PC-3 cells and lymphocytes, TAP1 augmented the expression of CD3⁺CD8⁺CD38⁺ T cell. Importantly, administration of Lentivirus TAP1 to prostate cancer cells in a xenograft mouse model can prolong survival and increase the CD4⁺ T cells, and CD8⁺ T cells as well as decrease Foxp3⁺ T cells in the tumor microenvironment. In summary, a recombinant lentivirus expressing TAP1 can effectively increase prostate cancer tumor-specific immune response.