Kinetic performance comparison of fully and superficially porous particles with a particle size of 5 碌m: Intrinsic evaluation and application to the impurity analysis of griseofulvin

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• 作者：Getu Kahsay^a ; Ken Broeckhoven^b ; Erwin Adams^a ; Gert Desmet^b ; Deirdre Cabooter^a ; ^{deirdre.cabooter@pharm.kuleuven.be" class="auth_mail}
• 关键词：Kinetic plots ; Superficially porous particles ; Loadability ; Griseofulvin ; 5  ; µ ; m particles
• 刊名：Talanta
• 出版年：May, 2014
• 年：2014
• 卷：122
• 期：Complete
• 页码：122-129
• 全文大小：1165 K

文摘

After the great commercial success of sub-3 碌m superficially porous particles, vendors are now also starting to commercialize 5 碌m superficially porous particles, as an alternative to their fully porous counterparts which are routinely used in pharmaceutical analysis. In this study, the performance of 5 碌m superficially porous particles was compared to that of fully porous 5 碌m particles in terms of efficiency, separation performance and loadability on a conventional HPLC instrument. Van Deemter and kinetic plots were first used to evaluate the efficiency and performance of both particle types using alkylphenones as a test mixture. The van Deemter and kinetic plots showed that the superficially porous particles provide a superior kinetic performance compared to the fully porous particles over the entire relevant range of separation conditions, when both support types were evaluated at the same operating pressure. The same observations were made both for isocratic and gradient analysis. The superior performance was further demonstrated for the separation of a pharmaceutical compound (griseofulvin) and its impurities, where a gain in analysis time of around 2 could be obtained using the superficially porous particles. Finally, both particle types were evaluated in terms of loadability by plotting the resolution of the active pharmaceutical ingredient and its closest impurity as a function of the signal-to-noise ratio obtained for the smallest impurity. It was demonstrated that the superficially porous particles show better separation performance for griseofulvin and its impurities without significantly compromising sensitivity due to loadability issues in comparison with their fully porous counterparts. Moreover these columns can be used on conventional equipment without modifications to obtain a significant improvement in analysis time.