Antimicrobial effects of commensal oral species are regulated by environmental factors

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• 作者：Esteban Rodriguez Herrero^a ; ^{esteban.rodriguezherrero@med.kuleuven.be" class="auth_mail" title="E-mail the corresponding author} ; Vera Slomka^a ; Kristel Bernaerts^b ; Nico Boon^c ; Emma Hernandez-Sanabria^c ; Bernardo Born Passoni^a ; Marc Quirynen^a ; ^d ; Wim Teughels^a ; ^d ; ^{wim.teughels@med.kuleuven.be" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bacteriocins ; Hydrogen peroxide ; Streptococcus ; Beneficial bacteria ; Periodontal pathogens ; Commensal bacteria
• 刊名：Journal of Dentistry
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：47
• 期：Complete
• 页码：23-33
• 全文大小：1190 K

文摘

The objectives of this study are to identify oral commensal species which can inhibit the growth of the main periodontopathogens, to determine the antimicrobial substances involved in these inhibitory activities and to evaluate the influence of environmental factors on the magnitude of these inhibitions.

Methods

The spotting technique was used to quantify the capacity of 13 commensal species to inhibit the growth of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia. By altering experimental conditions (distance between spots and size of spots and concentration of commensal and pathogen) as well as environmental factors (inoculation sequence, oxygen and nutrition availability) the influence of these factors was evaluated. Additionally, the mechanism of inhibition was elucidated by performing inhibition experiments in the presence of peroxidase, trypsin and pepsin and by evaluating acid production.

Results

Streptococcus sanguinis, Streptococcus cristatus, Streptococcus gordonii, Streptococcus parasanguinis, Streptococcus mitis and Streptococcus oralis significantly inhibit the growth of all pathogens. The volume of the spots and concentration of the commensal have a significant positive correlation with the amount of inhibition whereas distance between the spots and concentration of the pathogen reduced the amount of inhibition. Inhibition is only observed when the commensal species are inoculated 24 h before the pathogen and is more pronounced under aerobic conditions. Hydrogen peroxide production by the commensal is the main mechanism of inhibition.

Conclusion

Bacterial antagonism is species specific and depending on experimental as well as environmental conditions. Blocking hydrogen peroxide production neutralizes the inhibitory effect.

Clinical significance

Identifying beneficial oral bacteria and understanding how they inhibit pathogens might help to unravel the mechanisms behind dysbiotic oral diseases. In this context, this study points towards an important role for hydrogen peroxide. The latter might lead in the future to novel preventive strategies for oral health based on improving the antimicrobial properties of commensal oral bacteria.