Semiquinone glucoside derivative (SQGD) isolated from Bacillus sp. INM-1 protects against gamma radiation-induced oxidative stress

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• 作者：Saurabh Mishra^a ; Poonam Malhotra^a ; Ashutosh K. Gupta^a ; Praveen K. Singh^a ; Saleem Javed^b ; Raj Kumar^a ; ^b ; ^{rajkumar790@yahoo.com" class="auth_mail}
• 关键词：Antioxidant ; Oxidative stress ; Carbonylation ; Lipid-peroxidation ; Restriction enzyme ; Toxicity
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：March, 2014
• 年：2014
• 卷：37
• 期：2
• 页码：553-562
• 全文大小：2027 K

文摘

In the present study, radioprotective potential of Semiquinone glucoside derivative (SQGD) isolated from radioresistant bacterium Bacillus sp. INM-1 was evaluated. 纬-Radiation induced protein carbonylation, plasmid DNA damage, enzyme functional impairment, lipid peroxidation, HO radicals generation and their protection by SQGD was assessed. As a result of SQGD treatment, significant inhibition (p < 0.05) in protein carbonylation was observed with BSA. SQGD treatment was found to restore supercoiled (鈭?0 卤 3.21%) form of irradiated plasmid DNA against 纬-irradiation. SQGD protects enzymes (EcoR1 and BamH1) against radiation-induced dysfunctioning. SQGD significantly inhibited (p < 0.05) lipid peroxidation in liposomes, brain and liver homogenate. Higher HO^{鈥?/sup> radicals-averting activity of SQGD was observed in the serum and liver homogenate of C57BL/6 mice against H₂O₂-induced oxidative stress. In conclusion, SQGD demonstrates excellent radical-scavenging activity towards bio-macromolecules in irradiated environment and can be developed as an ideal radioprotector against radiation-induced oxidative stress in future.}