Supplementation of the fermented soy product ImmuBalance? effectively reduces itching behavior of atopic NC/Tnd mice

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• 作者：Akira Matsuda ; Akane Tanaka ; Weijun Pan ; Noriko Okamoto ; Kumiko Oida ; Nobuyuki Kingyo ; Yosuke Amagai ; Yan Xia ; Hyosun Jang ; Sho Nishikawa ; Naoki Kajiwara ; Ginnae Ahn ; Keitaro Ohmori ; Hiroshi Matsuda
• 关键词：Atopic dermatitis ; NC/Tnd mice ; Itch sensation ; Probiotics ; Fermented soy product
• 刊名：Journal of Dermatological Science
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：67
• 期：2
• 页码：130-139
• 全文大小：1320 K

文摘

Background

Effects of probiotics on the prevention of atopic diseases have been proposed recently. Although we have already reported the suppressive effects of the probiotic, ImmuBalance?, on a mouse model for peanuts allergy, its influence on atopic diseases remains unclear.

Objective

Potential efficacy of ImmuBalance?, which is the fermented soy product, on treatment of atopic dermatitis (AD) was investigated using a mouse model for human AD, NC/Tnd mice.

Methods

For in vivo study, ImmuBalance containing chow or a control diet were fed to NC/Tnd mice with moderate dermatitis for 2 weeks. Topical application of FK506 ointment was used as a positive control. Clinical skin severity scores, scratching behaviors, trans-epidermal water loss (TEWL), and histological features were analyzed. For in vitro study, suppressive effect of ImmuBalance? on nerve growth factor (NGF)-activated neurite outgrowth of PC12 cells was examined.

Results

Clinical skin severity scores of the mice fed with ImmuBalance containing chow were gradually reduced as well as the mice treated with FK506. Feeding with ImmuBalance completely inhibited the increase in scratching behavior of NC/Tnd mice. The value of TEWL of NC/Tnd mice fed with ImmuBalance was significantly decreased. In addition, histological examination revealed that application of ImmuBalance decreased the number of PGP9.5-positive neuronal fibers in the lesional skin. When ImmuBalance extract was added to the culture, NGF-activated neurite outgrowth of PC12 cells was diminished through the inhibition of the phosphatidylinositol 3-kinase phosphorylation.

Conclusion

ImmuBalance could exhibit favorable alterations on AD symptoms, particularly through down regulation of the itch sensation.