Caenorhabditis elegans p97/CDC-48 is crucial for progression of meiosis I
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- 作者：Yohei Sasagawa ; Kunitoshi Yamanaka ; Shingo Nishikori ; Teru Ogura
- 关键词：Meiotic division I ; Chromosome condensation ; AAA ATPase ; p97/VCP/Cdc48p ; C. elegans
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：6 July 2007
- 年：2007
- 卷：358
- 期：3
- 页码：920-924
- 全文大小：1446 K
文摘
p97/VCP/Cdc48p belongs to the AAA (ATPases associated with diverse cellular activities) family and has been indicated to be required for mitotic M-phase. We previously reported that simultaneous depletion of two p97 homologues, CDC-48.1 and CDC-48.2, in Caenorhabditis elegans caused the complete embryonic lethality, and that a large number of vacuole-like structures were observed in the dead embryos. However, cellular functions of p97 in embryogenesis have not been revealed. In this study, we analyzed effects of p97 depletion on meiotic progression. Simultaneous depletion of both p97 resulted in the formation of aberrant multinucleate cells and sometimes ectopic furrows in embryos. Importantly, meiotic chromosomes were not divided at meiotic metaphase I in p97-depleted embryos, although spindle formation and disassembly occurred. Furthermore, we found that chromosome condensation was significantly reduced in p97-depleted oocytes. Taken these results altogether, we propose that C. elegans p97 plays an important role in the progression of meiosis.