Dentin remineralization in acid challenge environment via PAMAM and calcium phosphate composite

详细信息    查看全文

• 作者：Kunneng Liang^a ; ^b ; Michael D. Weir^b ; Xianju Xie^b ; ^c ; Lin Wang^b ; ^d ; Mark A. Reynolds^b ; Jiyao Li^a ; ^{jiyaoliscu@163.com" class="auth_mail" title="E-mail the corresponding author} ; Hockin H.K. Xu^b ; ^e ; ^f ; ^{hxu@umaryland.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dentin remineralization ; PAMAM ; Composite ; Acid challenge ; Demineralization ; Calcium phosphate nanoparticles
• 刊名：Dental Materials
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：32
• 期：11
• 页码：1429-1440
• 全文大小：5094 K

文摘

The objective of this study was to investigate the effects of poly (amido amine) (PAMAM), composite with nanoparticles of amorphous calcium phosphate (NACP), and the combined PAMAM + NACP nanocomposite treatment, on remineralization of demineralized dentin in a cyclic artificial saliva/lactic acid environment for the first time.

Methods

r0040">Dentin specimens were prepared and demineralized with 37% phosphoric acid for 15 s. Four groups were prepared: (1) dentin control, (2) dentin coated with PAMAM, (3) dentin with NACP composite, (4) dentin with PAMAM + NACP. Specimens were treated with a cyclic artificial saliva/lactic acid regimen for 21 days. Acid neutralization and calcium (Ca) and phosphate (P) ion concentrations were measured. The remineralized dentin specimens were examined by scanning electron microscopy (SEM) and hardness testing.

Results

r0045">NACP nanocomposite had mechanical properties similar to commercial control composites (p > 0.1). NACP composite had acid-neutralization and Ca and P ion release capability. PAMAM or NACP composite each alone achieved remineralization and increased the hardness of demineralized dentin (p < 0.05). PAMAM + NACP nanocomposite achieved the greatest mineral regeneration in demineralized dentin and the greatest hardness increase in demineralized dentin, which approached the hardness of healthy dentin (p > 0.1).

Significance

r0050">The superior remineralization efficacy of PAMAM + NACP was demonstrated for the first time. PAMAM + NACP induced remineralization in demineralized dentin in an acid challenge environment, when conventional remineralization methods such as PAMAM did not work well. The novel PAMAM + NACP composite approach is promising for a wide range of dental applications to inhibit caries and protect tooth structures.