Evaluation of multiple electrode aggregometry in whole blood using Multiplate? Mini Test cells

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• 作者：Kurtis R. Lee^a ; ^{kurtis.lee2@UHBristol.nhs.uk} ; Veerle J.E. Verheyden^a ; Andrew D. Mumford^b
• 关键词：Platelet function testing ; Electrical impedance aggregometry ; Point-of-care ; Mini test cells
• 刊名：Thrombosis Research
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：129
• 期：4
• 页码：e59-e64
• 全文大小：633 K

文摘

Multiple electrode aggregometry (MEA) enables rapid platelet function testing in whole blood using 600 ¦ÌL disposable standard test cells (STC). However, newly available 350¦ÌL mini test cells (MTC) could potentially be advantageous in some clinical settings where sample volume is limiting. In order to evaluate the diagnostic performance of MTC, we have estimated assay imprecision, correlation and agreement between area under curve (AUC) determined using MTC and STC in whole blood from healthy donors and from 119 cardiac surgery patients. Imprecision was similar with ADP, AA and TRAP test reagents using STC and MTC, but was markedly higher with the unvalidated ADR reagent. AUC determined using MTC and STC and the ADP, AA and TRAP reagents correlated strongly although MTC yield consistently lower AUC values reflecting fewer platelets in the smaller test cell. Agreement between AUC from STC and MTC was less strong, probably reflecting a composite effect of imprecision from both assay formats. MTC and STC are equally valid for MEA but AUC values obtained using one test format cannot be directly transformed to the other. Therefore, STC and MTC cannot be used interchangeably and AUC results must be compared to separately determined reference intervals.