Atypical behaviour and survival of Streptococcus pyogenes L forms during intraperitoneal infection in rats

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• 作者：Michailova ; Lilia ; Markova ; Nadya ; Radoucheva ; Tatyana ; Stoitsova ; Stoyanka ; Kussovski ; Vesselin ; et. al.
• 关键词：Streptococcus pyogenes ; Protoplast L form ; Phagocytosis ; Peritoneal exudate cell ; Electron microscopy
• 刊名：FEMS Immunology and Medical Microbiology
• 出版年：2000
• 出版时间：May, 2000
• 年：2000
• 卷：28
• 期：1
• 页码：55-65
• 全文大小：2.96 M

文摘

Groups of rats were injected intraperitoneally with cell wall-deficient (L) forms of Streptococcus pyogenes, with their parental (S) forms, as well as with a combined inoculum of both forms (S+L). Peritoneal exudate samples were harvested on days 1, 3, 7, 15 and 30 after challenge and were investigated by microbiological, electron microscopic, cytometric and biochemical methods. Parental S forms were isolated from peritoneal exudate samples up to day 15 post infection, while L form cultures were isolated until the end of the examined interval. Electron microscopic examination revealed continuous adhesion of L forms on the macrophage surface as well as intracellular persistence inside them. It was demonstrated that the intraperitoneal inflammatory response to L form infection was higher than to the other infections and the monocyte-macrophage populations were predominant. The established atypical behaviour and long survival of S. pyogenes L forms in the rat’s peritoneum could explain some of the mechanisms of the pathogens’ persistence as well as the reasons for chronic streptococcal infections.