Intermolecular relations between the glucocorticoid receptor, ZAP-70 kinase, and Hsp-90

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• 作者：Domokos Bartis ; Ferenc Boldizsá ; r ; Krisztiá ; n Kvell ; Mariann Szabó ; Lá ; szló ; Pá ; linká ; s ; Pé ; ter Né ; meth ; É ; va Monostori ; Tí ; mea Berki
• 关键词：ZAP-70 ; Glucocorticoid receptor (GR) ; Hsp-90 ; Non-genomic glucocorticoid effects ; Jurkat cells
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：2 March 2007
• 年：2007
• 卷：354
• 期：1
• 页码：253-258
• 全文大小：2008 K

文摘

The glucocorticoid receptor (GR) participates in both genomic and non-genomic glucocorticoid hormone (GC) actions by interacting with other cytoplasmic signalling proteins. Previously, we have shown that high dose Dexamethasone (DX) treatment of Jurkat cells causes tyrosine phosphorylation of ZAP-70 within 5 min in a GR-dependent manner. By using co-immunoprecipitation and confocal microscopy, here we demonstrate that the liganded GR physically associates with ZAP-70, in addition to its phosphorylation changes. The association of the ligand-bound GR and ZAP-70 was also observed in HeLa cells transfected with ZAP-70, suggesting that this co-clustering is independent of lymphocyte specific factors. Furthermore, the ZAP-70 was found to also co-precipitate with Hsp-90 chaperone both in Jurkat and transgenic HeLa cells, independent of the presence of DX. These findings raise the possibility that ZAP-70 may serve as an important link between GC and TcR-induced signaling, thereby transmitting non-genomic GC action in T-cells.