Suppression of interleukin-2 gene expression by isoeugenol is mediated through down-regulation of NF-AT and NF-κB

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• 作者：Kyung-Ran Park ; Jung-Hee Lee ; ChunYeon Choi ; Kwang-Hyeon Liu ; Dae-Hyun Seog ; Yong-Ho Kim ; Dong-Eun Kim ; Chul-Ho Yun ; Sung Su Yea
• 关键词：Isoeugenol ; Interleukin-2 ; NF-AT ; NF-κ ; B
• 刊名：International Immunopharmacology
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：7
• 期：9
• 页码：1251-1258
• 全文大小：596 K

文摘

Isoeugenol is a naturally occurring methoxyphenol found in a variety of foods and essential oils. We investigated the effect of isoeugenol on T-cell function and the regulatory mechanism underlying its effect. Isoeugenol and its structural analog eugenol suppressed the lymphoproliferative response to concanavalin A stimulation in B6C3F1 mouse splenocyte cultures. Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. To further characterize the inhibitory mechanism of isoeugenol at the transcriptional level, we examined the DNA binding activity of the transcription factors for IL-2 using an electrophoretic mobility shift assay. Isoeugenol decreased the binding activity of NF-AT and NF-κB in PMA/Io-stimulated EL4.IL-2 cells, but no significant effect was observed for AP-1 or Oct binding activity. Western blot analysis showed that isoeugenol also decreased the nuclear translocation of cytoplasmic NF-AT and NF-κB. These results suggest that isoeugenol suppresses IL-2 production through a decrease of IL-2 mRNA expression and that the inhibition is mediated, at least in part, through the down-regulation of NF-AT and NF-κB.