Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities
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- 作者：Eun Sung Jung ; Sarah Lee ; Sun-Hyung Lim ; Sun-Hwa Ha ; Kwang-Hyeon Liu ; Choong Hwan Lee
- 关键词：Oryza sativa ; LED lights ; Metabolite profiling ; GC&ndash ; IT-MS ; UPLC&ndash ; Q-TOF-MS ; Antioxidant activity
- 刊名：Plant Science
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：210
- 期：Complete
- 页码：61-69
- 全文大小：2289 K
文摘
Metabolite profiling of rice leaves (Oryza sativa cv. Ilmi) was performed to investigate the short-term responses to different light-emitting diode (LED) lights, blue (B), green (G), red (R), white (W), shade (S), by using gas chromatography-ion trap-mass spectrometry (GC-IT-MS) and ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) with multivariate analysis. Clear grouping patterns of each light-grown sample, except G and W, were shown in partial least squares-discriminant analysis (PLS-DA). Thirty-two primary metabolites and eleven secondary metabolites were selected and visualized using heatmap. Antioxidant activities of rice leaves followed the order B = W = G > R > S and isoorientin-2¡ä¡ä-O-glucoside, isovitexin-2¡ä¡ä-O-glucoside, isoorientin-2¡ä¡ä-O-(6¡ä¡ä¡ä-¦Ñ-coumaroyl)-glucoside, and isoscoparin-2¡ä¡ä-O-glucoside showed similar relative differences and had higher Pearson's correlation coefficients than other metabolites in correlation network. According to the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) between B and R, the levels of amino acids, organic acids, fatty acids, and flavonoid glycosides were relatively high in B, whereas the glucose and fructose levels were high in R.