Surface plasmon resonance spectroscopic chiral discrimination using self-assembled leucine derivative monolayer

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• 作者：Hongxia Chen ; Haiyun Cheng ; Jaebeom Lee ; Jae-Ho Kim ; Myung Ho Hyun ; Kwangnak Koh
• 关键词：Chiral differential detection ; Leucine ; Chiral stationary phase (CSP) ; Surface plasmon resonance (SPR) ; Self-assembled monolayer (SAM)
• 刊名：Talanta
• 出版年：2008
• 出版时间：30 June 2008
• 年：2008
• 卷：76
• 期：1
• 页码：49-53
• 全文大小：214 K

文摘

Pirkle-type chiral stationary phases (CSPs) showed excellent enantiomeric separation for amino acid derivatives by forming energetically different two transient diastereomeric π–π donor–acceptor complexes with two enantiomers. A CSP derived from N-(3,5-dinitrobenzoyl) leucine with a thiol ending group for immobilization on Au was synthesized and self-assembled on Au surface as chiral sensing layer. The monolayer characterized by spectroscopic and microscopic methods such as AFM, FTIR reflection absorption spectroscopy (FTIR-RAS) and cyclic voltammetry (CV). The enantiospecific detection onto CSP of the leucine derivative was studied by surface plasmon resonance (SPR). (S)-CSP SAM showed high chiral differential detection for (S)-analyte in a range of 1.0 × 10⁻⁹ to 1.0 × 10⁻⁴ M. In combination with the SPR method, the leucine derivative monolayer provided a reliable and simple experimental platform for enantiospecific detection.