Effects of feeding a diet containing Gymnema sylvestre extract: Attenuating progression of obesity in C57BL/6J mice

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• 作者：Hyeon-Jeong Kim¹ ; Seong-Ho Hong¹ ; Seung-Hee Chang¹ ; Sanghwa Kim¹ ; ² ; Ah Young Lee¹ ; Yoonjeong Jang¹ ; Orkhonselenge Davaadamdin¹ ; Kyeong-Nam Yu¹ ; Ji-Eun Kim¹ ; Myung-Haing Cho¹ ; ² ; ³ ; ⁴ ; ⁵ ; ^{mchotox@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gymnema sylvestre extract ; Obesity ; Adipocyte ; Body weight gain ; High-fat diet ; Liver injury
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：9
• 期：5
• 页码：437-444
• 全文大小：1624 K

文摘

To investigate the effect of Gymnema sylvestre extract (GS) on initial anti-obesity, liver injury, and glucose homeostasis induced by a high-fat diet (HFD).

Methods

The dry powder of GS was extracted with methanol, and gymnemic acid was identified by high performance liquid chromatography as deacyl gymnemic acid. Male C57BL/6J mice that fed on either a normal diet, normal diet containing 1 g/kg GS (CON+GS), HFD, or HFD containing 1.0 g/kg GS (HFD + GS) for 4 weeks were used to test the initial anti-obesity effect of GS. Body weight gain and food intake, and serum levels about lipid and liver injury markers were measured. Histopathology of adipose tissue and liver stained with hematoxylin and eosin (H&E) and oil-red O were analyzed. After 4 weeks of GS extract feeding, intraperitoneal glucose tolerance test (IPGTT) was performed.

Results

The methanol extracts of GS exerted significant anti-obesity effects in HFD + GS group. They decreased body weight gain, a lower food and energy efficiency ratio, and showed lower serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL)-cholesterol, very-low density lipoprotein (VLDL)-cholesterol and leptin compared with the HFD group. The decreases of abdominal as well as epididymal fat weight and adipocyte hypertrophy, lipid droplets in liver, and serum levels of aspartate aminotransferase (AST) and alanine transaminase (ALT) were also observed. The CON + GS group showed an effect of glucose homeostasis compared to the CON group.

Conclusions

This study shows that GS provide the possibility as a key role in an initial anti-obesity effects feeding with a HFD.