Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase
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- 作者：Hiroyuki Okano
- 关键词：MMLV ; Moloney murine leukemia virus ; RT ; reverse transcriptase
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：111-120
- 全文大小：1860 K
文摘
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We devised a novel cDNA synthesis reaction using RT and other two enzymes.
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One is a Thermotoga petrophila family A DNA polymerase variant, K4pol
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The other is a Thermococcus kodakarensis helicase, Tk-EshA.
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The reaction condition was optimized using Taguchi’s statistical method.
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As a result, 100 to 1000-fold higher sensitivity was achieved than current method.