Toward the production of flavone-7-O-尾-d-glucopyranosides using Arabidopsis glycosyltransferase in Escherichia coli

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• 作者：Nguyen Huy Thuan ; Je Won Park ; Jae Kyung Sohng
• 关键词：Escherichia coli ; Flavones ; Glucosylation ; Heterologous production ; Whole cell biotransformation
• 刊名：Process Biochemistry
• 出版年：November, 2013
• 年：2013
• 卷：48
• 期：11
• 页码：1744-1748
• 全文大小：953 K

文摘

Flavonoid glycosides are highly attractive targets due to their dominant roles in clinical, cosmetic production and in the food industry. In this research, an Escherichia coli strain bearing the reconstructed uridine-diphosphate glucose (UDP-glucose) pathway cassette and a putative glycosyltransferase from Arabidopsis thaliana, was developed as a host for the production of apigenin-7-O-尾-d-glucoside (APG) and baicalein-7-O-尾-d-glucoside (BCG) from exogenously supplied flavone aglycones (apigenin and baicalein, respectively). In order to improve the yield, genetic engineering of E. coli strains for optimization of intracellular UDP-glucose generation, as well as media optimization were carried out. The production was scaled up using a fed batch fermentation, and the maximal yield of products reached 90.88 渭M (39.28 mg L^鈭?) and 76.82 渭M (33.19 mg L^鈭?) of APG and BCG, respectively. And, the maximum bioconversion rate corresponded to 90.88% and 76.82% of apigenin and baicalein, respectively.