Wnt5a activates THP-1 monocytic cells via a ¦Â-catenin-independent pathway involving JNK and NF-¦ÊB activation
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- 作者：Jungtae Kim ; Wookyoung Chang ; Yusun Jung ; Kyuyoung Song ; Inchul Lee
- 关键词：FBS ; fetal bovine serum ; FHC ; ferritin heave chain ; HAECs ; human aortic endothelial cells ; IFN ; interferon ; IKK ; IkB kinase ; JNK ; c-Jun N-terminus kinase ; LPS ; lipopolysaccharide ; MAPK ; mitogen-activated protein kinase ; MKK ; mitogen-activated-protein kina
- 刊名：Cytokine
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：60
- 期：1
- 页码：242-248
- 全文大小：780 K
文摘
Wnt5a has been implicated in the activation of macrophages. However, the profile and mechanism of downstream regulation has not been characterized. In this study, we have investigated the regulation of Wnt5a-induced activation in monocytic THP-1 cells. Wnt5a activated THP-1 cells, enhancing adhesion to endothelial cells. Hypoxia induced the production of Wnt5a, suggesting a role in the hypoxia-induced activation of macrophages. Wnt5a induced the expression of various pro-inflammatory cytokines and inflammatory mediators, particularly IL8 and CXCL2, suggesting a major role in the secretion of CXC chemokines by macrophages. Wnt5a induced JNK phosphorylation and NF-¦ÊB activation via ¦Â-catenin-independent signaling. Interestingly, SP600125, a specific inhibitor of JNK, inhibited Wnt5a-induced activation of NF-¦ÊB, supporting JNK-dependent NF-¦ÊB activation. Our data suggest that Wnt5a activates monocytic cells via JNK and NF-¦ÊB activation.