Antimicrobial Resistance, Virulence Profile, and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China, 2013-2014

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• 作者：Shao Fei YANAuthor Vitae ; Wei WANG ; Li BAI ; Yu Jie HU ; Yin Ping DONG ; Jin XU ; Feng Qin LIp> ; p>p>lifengqin@cfsa.net.cn" class="auth_mail" title="E-mail the corresponding authorp>
• 刊名：Biomedical and Environmental Sciences
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：29
• 期：6
• 页码：448-452
• 全文大小：432 K

文摘

We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China. Antimicrobial resistance was determined by broth microdilution following the Clinical and Laboratory Standards Institute protocol. Molecular serotyping, virulence, and resistance genes were identified using PCR. Multi-locus sequence typing was performed on resistant strains. A total of 11.53% (113/980) isolates were resistant, from which 82.3% (93/113) harbored all the virulence genes tested. The resistant strains were subtyped into 18 sequence types (STs), from which ST2, ST5, ST8, and ST9 were involved in listeriosis. This study indicated that several L. monocytogenes isolates from ready-to-eat foods in China have pathogenic potential and are resistant to antibiotics, including antibiotics used as medicines by humans for listeriosis treatment.