Brassinolides and IAA induce the transcription of four α-expansin genes related to development in Cicer arietinum
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- 作者：Sá ; nchez ; M< ; ce ; sup> ; a< ; /ce ; sup> ; Angeles ; Mateos ; Isabel ; Labrador ; Emilia ; et. al.
- 关键词：Brassinolides ; Cell wall ; Cicer arietinum ; Epicotyl ; Expansin ; Growth ; ABA ; abscisic acid ; BR ; brassinolides ; IAA ; indole acetic acid ; GA ; gibberellin
- 刊名：Plant Physiology and Biochemistry
- 出版年：2004
- 出版时间：September, 2004
- 年：2004
- 卷：42
- 期：9
- 页码：709-716
- 全文大小：519 K
文摘
Four different cDNAs encoding α-expansins have been identified in Cicer arietinum (Ca-EXPA1, Ca-EXPA2, Ca-EXPA3 and Ca-EXPA4). The shared amino acid sequence similarity among the four α-expansin proteins ranged from 67 to 89 % . All of them display common characteristics such as molecular mass (around 24 kDa), amino acid numbers, and also the presence of a signal peptide. The transcription pattern of chickpea α-expansin genes in seedlings and plants suggests a specific role for each of the four α-expansins in different phases of development or in different plant organs. High levels of Ca-EXPA2 transcripts coincide with maximum epicotyl and stem growth, indicating an important involvement of this particular α-expansin in elongating tissues. Ca-EXPA3 would be related to radicle development, while Ca-EXPA4 seems to be involved in pod development. A considerable increase in the level of all Ca-EXPA transcripts accompanied the indole acetic acid (IAA) plus brassinolide (BR)-induced elongation of excised epicotyl segments. This IAA + BR induction was seen even for the chickpea expansin genes whose transcription was not affected by IAA or BR alone.